The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor
Identifieur interne : 004537 ( Main/Exploration ); précédent : 004536; suivant : 004538The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor
Auteurs : He Wang [États-Unis] ; Alan M. Lambowitz [États-Unis]Source :
- Cell [ 0092-8674 ] ; 1993.
English descriptors
- Teeft :
- Akins, Alkaline phosphatase, Biol, Cdna, Cdna primers, Cdna product, Cdna synthesis, Clone, Dgmp, Dgtp, Digestion, Eickbush, England biolabs, Experimental procedures, Initiation, Initiation reaction, Initiation site, Internal site, Intron, Kennell, Kinase, Kuiper, Lambowitz, Ligated, Mauriceville, Mauriceville plasmid, Mauriceville plasmid enzyme, Mitochondrial, Mitochondrion, Molecular weight markers, Neurospora, Nsil, Nucleotide, Oligonucleotide, Oligonucleotides, Pbluescript, Penultimate, Phosphatase, Plasmid, Plasmid transcript, Polyacrylamide, Polymerase, Polynucleotide, Polynucleotide kinase, Prime cdna synthesis, Primer, Protease, Reaction medium, Replication, Retroelements, Rnaase, Sau3al fragments, Standard reaction medium, Strand synthesis, Template, Transcript, Transcriptase, Transcription, Transcription reactions, Trna, Unpublished data, Urea, Wang.
Abstract
Abstract: We show that the reverse transcriptase (RT) encoded by the Mauriceville mitochondrial plasmid of Neurospora closely resembles viral RNA-dependent RNA polymerases in initiating cDNA synthesis opposite the penultimate C residue of a 3′ tRNA-like structure and has the unprecedented ability for a DNA polymerase to initiate DNA synthesis at a specific site in a natural template without a primer. The Mauriceville plasmid enzyme can also use DNA or RNA primers in a manner suggesting how a primitive RT could have evolved from an RNA-dependent RNA polymerase into retroviral and other types of RTs. The characteristics of the Mauriceville plasmid RT suggest that it may be related to the progenitor of present-day RTs and DNA polymerases.
Url:
DOI: 10.1016/0092-8674(93)90317-J
Affiliations:
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The Mauriceville plasmid enzyme can also use DNA or RNA primers in a manner suggesting how a primitive RT could have evolved from an RNA-dependent RNA polymerase into retroviral and other types of RTs. The characteristics of the Mauriceville plasmid RT suggest that it may be related to the progenitor of present-day RTs and DNA polymerases.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Ohio</li></region></list><tree><country name="États-Unis"><region name="Ohio"><name sortKey="Wang, He" sort="Wang, He" uniqKey="Wang H" first="He" last="Wang">He Wang</name></region><name sortKey="Lambowitz, Alan M" sort="Lambowitz, Alan M" uniqKey="Lambowitz A" first="Alan M." last="Lambowitz">Alan M. Lambowitz</name><name sortKey="Lambowitz, Alan M" sort="Lambowitz, Alan M" uniqKey="Lambowitz A" first="Alan M." last="Lambowitz">Alan M. Lambowitz</name><name sortKey="Lambowitz, Alan M" sort="Lambowitz, Alan M" uniqKey="Lambowitz A" first="Alan M." last="Lambowitz">Alan M. Lambowitz</name><name sortKey="Lambowitz, Alan M" sort="Lambowitz, Alan M" uniqKey="Lambowitz A" first="Alan M." last="Lambowitz">Alan M. Lambowitz</name><name sortKey="Wang, He" sort="Wang, He" uniqKey="Wang H" first="He" last="Wang">He Wang</name><name sortKey="Wang, He" sort="Wang, He" uniqKey="Wang H" first="He" last="Wang">He Wang</name><name sortKey="Wang, He" sort="Wang, He" uniqKey="Wang H" first="He" last="Wang">He Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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